Anti-Escherichia coli eae/Intimin Polyclonal Antibody (JN084014)

Name: Anti-Escherichia coli eae/Intimin Polyclonal Antibody
Brand: abinScience
SKU: JN084014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN084014

Reactividad de especies

Escherichia coli

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Escherichia coli eae/Intimin (Lys450-Gln655).

Objetivo

Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939

Purificación

Purified by antigen affinity column.

Número de acceso

P19809

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Intimin (eae) is a ~102 kDa protein. An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells. Necessary for the production of attaching and effacing lesions on infected human tissue culture cells. Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable).

1. Leo, JC. et al. (2015) Molecular microbiology 95, 80-100. PMID: 25353290

2. Weikum, J. et al. (2020) Scientific reports 10, 21249. PMID: 33277518

3. Jerse, AE. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7839-43. PMID: 2172966

4. Batchelor, M. et al. (2000) The EMBO journal 19, 2452-64. PMID: 10835344

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-EAE polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Escherichia coli eae/Intimin (Catalog No: JN084012)

Referencias

Datasheet

Anti-Escherichia coli eae/Intimin Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo