Escherichia coli eae/Intimin Recombinant Protein (N-His) (JN084012)

Name: Escherichia coli eae/Intimin Recombinant Protein (N-His)
Brand: abinScience
SKU: JN084012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN084012
Description	Recombinant Escherichia coli eae/Intimin Protein, N-His (JN084012) expressed in E. coli, spanning Lys450-Gln655. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P19809
Protein length	Lys450-Gln655
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Escherichia coli
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.15 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939
Background	Intimin (eae) is a ~102 kDa protein. An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells. Necessary for the production of attaching and effacing lesions on infected human tissue culture cells. Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable). 1. Leo, JC. et al. (2015) Molecular microbiology 95, 80-100. PMID: 25353290 2. Weikum, J. et al. (2020) Scientific reports 10, 21249. PMID: 33277518 3. Jerse, AE. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7839-43. PMID: 2172966 4. Batchelor, M. et al. (2000) The EMBO journal 19, 2452-64. PMID: 10835344
Note	For research use only.

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SDS-PAGE for Recombinant Escherichia coli eae/Intimin Protein, N-His

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JN084012-SDS-PAGE-1.jpg

Datasheet

Escherichia coli eae/Intimin Recombinant Protein (N-His).pdf

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Escherichia coli eae/Intimin Recombinant Protein (N-His) (JN084012)

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