Anti-Escherichia coli eae/Intimin Polyclonal Antibody (JN084014)

Name: Anti-Escherichia coli eae/Intimin Polyclonal Antibody
Brand: abinScience
SKU: JN084014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

JN084014

Species reactivity

Escherichia coli

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Escherichia coli eae/Intimin (Lys450-Gln655).

Target

Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939

Purification

Purified by antigen affinity column.

Accession

P19809

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Intimin (eae) is a ~102 kDa protein. An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells. Necessary for the production of attaching and effacing lesions on infected human tissue culture cells. Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable).

1. Leo, JC. et al. (2015) Molecular microbiology 95, 80-100. PMID: 25353290

2. Weikum, J. et al. (2020) Scientific reports 10, 21249. PMID: 33277518

3. Jerse, AE. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7839-43. PMID: 2172966

4. Batchelor, M. et al. (2000) The EMBO journal 19, 2452-64. PMID: 10835344

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EAE polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Escherichia coli eae/Intimin (Catalog No: JN084012)

References

Datasheet

Anti-Escherichia coli eae/Intimin Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Escherichia coli eae/Intimin Polyclonal Antibody (JN084014)

Overview

Images

References

Datasheet

SDS

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