Anti-Escherichia coli eae/Intimin Polyclonal Antibody (JN084014)

Name: Anti-Escherichia coli eae/Intimin Polyclonal Antibody
Brand: abinScience
SKU: JN084014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

JN084014

Реактивность видов

Escherichia coli

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Escherichia coli eae/Intimin (Lys450-Gln655).

Цель

Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939

Очистка

Purified by antigen affinity column.

Номер доступа

P19809

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Intimin (eae) is a ~102 kDa protein. An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells. Necessary for the production of attaching and effacing lesions on infected human tissue culture cells. Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable).

1. Leo, JC. et al. (2015) Molecular microbiology 95, 80-100. PMID: 25353290

2. Weikum, J. et al. (2020) Scientific reports 10, 21249. PMID: 33277518

3. Jerse, AE. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7839-43. PMID: 2172966

4. Batchelor, M. et al. (2000) The EMBO journal 19, 2452-64. PMID: 10835344

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-EAE polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Escherichia coli eae/Intimin (Catalog No: JN084012)

Ссылки

Даташит

Anti-Escherichia coli eae/Intimin Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа