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Anti-G protein/Gαβγ Recombinant Antibody (scFv16) (HX948023)

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Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHX948023
Реактивность видовHuman
ПримененияCrystallography, Cryo-EM
КлоональностьMonoclonal
ИзотипscFv-His
ID клонаscFv16
Цель Adenylate cyclase-stimulating G alpha protein, EC:3.6.5.-, GNAS, GNAS1, GSP, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P63092
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Gαβγ) is a ~45 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins.

1. Pak, Y. et al. (2002) Molecular and cellular biology 22, 7942-52. PMID: 12391161
2. Gao, X. et al. (2007) The Journal of biological chemistry 282, 294-302. PMID: 17110384
3. Thiele, S. et al. (2011) Human mutation 32, 653-60. PMID: 21488135
4. Brand, CS. et al. (2015) Molecular pharmacology 88, 758-67. PMID: 26206488
5. Farfel, Z. et al. (1996) The Journal of biological chemistry 271, 19653-5. PMID: 8702665
6. Warner, DR. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4268-72. PMID: 10200251
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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