Anti-GNAS Polyclonal Antibody (HX948014)

Name: Anti-GNAS Polyclonal Antibody
Brand: abinScience
SKU: HX948014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX948014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GNAS (Met1-Leu394).

Target

GNAS1, GNAS, GSP, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein

Purification

Purified by antigen affinity column.

Accession

P63092

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (GNAS) is a ~45 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins.

1. Pak, Y. et al. (2002) Molecular and cellular biology 22, 7942-52. PMID: 12391161

2. Gao, X. et al. (2007) The Journal of biological chemistry 282, 294-302. PMID: 17110384

3. Thiele, S. et al. (2011) Human mutation 32, 653-60. PMID: 21488135

4. Brand, CS. et al. (2015) Molecular pharmacology 88, 758-67. PMID: 26206488

5. Farfel, Z. et al. (1996) The Journal of biological chemistry 271, 19653-5. PMID: 8702665

6. Warner, DR. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4268-72. PMID: 10200251

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GNAS polyclonal antibody at 1ug/mL on recombinant GNAS (Catalog No. HX948012).

References

Datasheet

Anti-GNAS Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GNAS Polyclonal Antibody (HX948014)

Overview

Images

References

Datasheet

SDS

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