Anti-G protein Recombinant Nanobody (Nb35) (HC114013)

Name: Anti-G protein Recombinant Nanobody (Nb35)
Brand: abinScience
SKU: HC114013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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SDS

Vista general

Número de catálogo	HC114013
Descripción	Anti-G protein Nanobody (Nb35) [Nb35] (HC114013) is a alpaca monoclonal antibody detecting G protein in Cyro-EM, X-ray Crystallography. Suitable for Human.
Reactividad de especies	Human
Aplicaciones	Cyro-EM, X-ray Crystallography
Especie huésped	Alpaca
Clonalidad	Monoclonal
Isotipo	VHH-His
ID de clon	Nb35
Objetivo	G protein
Nivel de endotoxinas	<0.01 EU/ug
Pureza	>95% as determined by SDS-PAGE.
Purificación	Purified by Nickel column.
Número de acceso	P63092
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Nanobody 35, Nanobody-35, Nb-35
Fondo	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short is a ~45 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. 1. Pak, Y. et al. (2002) Molecular and cellular biology 22, 7942-52. PMID: 12391161 2. Gao, X. et al. (2007) The Journal of biological chemistry 282, 294-302. PMID: 17110384 3. Thiele, S. et al. (2011) Human mutation 32, 653-60. PMID: 21488135 4. Brand, CS. et al. (2015) Molecular pharmacology 88, 758-67. PMID: 26206488 5. Farfel, Z. et al. (1996) The Journal of biological chemistry 271, 19653-5. PMID: 8702665 6. Warner, DR. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4268-72. PMID: 10200251
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Anti-G protein Nanobody (Nb35)

Referencias

Datasheet

Anti-G protein Recombinant Nanobody (Nb35).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

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