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Anti-G protein Recombinant Nanobody (Nb35) (HC114013)

Anti-G protein Recombinant Nanobody (Nb35)
Anti-G protein Recombinant Nanobody (Nb35)
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Spécification :
  • 50ug
  • 100ug
  • 1mg
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  • SDS

Aperçu
Numéro de catalogueHC114013
Description
Anti-G protein Nanobody (Nb35) [Nb35] (HC114013) is a alpaca monoclonal antibody detecting G protein in Cyro-EM, X-ray Crystallography. Suitable for Human.
Reactivité des espècesHuman
ApplicationsCyro-EM, X-ray Crystallography
Espèce hôteAlpaca
ClonalitéMonoclonal
IsotypeVHH-His
ID de cloneNb35
Cible G protein
Niveau d'endotoxines <0.01 EU/ug
Pureté >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Numéro d'accès P63092
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsNanobody 35, Nanobody-35, Nb-35
Contexte

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short is a ~45 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins.

1. Pak, Y. et al. (2002) Molecular and cellular biology 22, 7942-52. PMID: 12391161
2. Gao, X. et al. (2007) The Journal of biological chemistry 282, 294-302. PMID: 17110384
3. Thiele, S. et al. (2011) Human mutation 32, 653-60. PMID: 21488135
4. Brand, CS. et al. (2015) Molecular pharmacology 88, 758-67. PMID: 26206488
5. Farfel, Z. et al. (1996) The Journal of biological chemistry 271, 19653-5. PMID: 8702665
6. Warner, DR. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4268-72. PMID: 10200251
Note For research use only
Images
  • Anti-G protein Recombinant Nanobody (Nb35)

    SDS-PAGE

    SDS-PAGE for Anti-G protein Nanobody (Nb35)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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