Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

Name: Anti-GM130/GOLGA2 Polyclonal Antibody
Brand: abinScience
SKU: HC396014
Price: 96 USD
Availability: InStock

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HC396014

Descripción

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014) is a rabbit polyclonal antibody detecting GOLGA2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human GM130/GOLGA2 (Ala361-Ala678).

Objetivo

130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, Golgin subfamily A member 2, GOLGA2

Purificación

Purified by antigen affinity column.

Número de acceso

Q08379

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Golgin subfamily A member 2 (GM130/GOLGA2) is a ~113 kDa protein. Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization.

1. Puthenveedu, MA. et al. (2006) Nature cell biology 8, 238-48. PMID: 16489344

2. Wei, JH. et al. (2015) Cell 162, 287-299. PMID: 26165940

3. Rivero, S. et al. (2009) The EMBO journal 28, 1016-28. PMID: 19242490

4. Kodani, A. et al. (2008) Molecular biology of the cell 19, 745-53. PMID: 18045989

5. Kodani, A. et al. (2009) Molecular biology of the cell 20, 1192-200. PMID: 19109421

6. Marra, P. et al. (2007) Molecular biology of the cell 18, 1595-608. PMID: 17314401

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-GOLGA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate

Referencias

Datasheet

Anti-GM130/GOLGA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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