Human GM130/GOLGA2 Recombinant Protein (N-His) (HC396012)

Name: Human GM130/GOLGA2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC396012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC396012
Description	Recombinant Human GM130/GOLGA2 Protein, N-His (HC396012) expressed in E. coli, spanning Ala361-Ala678. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q08379
Protein length	Ala361-Ala678
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.04 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, Golgin subfamily A member 2, GOLGA2
Background	Golgin subfamily A member 2 (GM130/GOLGA2) is a ~113 kDa protein. Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization. 1. Puthenveedu, MA. et al. (2006) Nature cell biology 8, 238-48. PMID: 16489344 2. Wei, JH. et al. (2015) Cell 162, 287-299. PMID: 26165940 3. Rivero, S. et al. (2009) The EMBO journal 28, 1016-28. PMID: 19242490 4. Kodani, A. et al. (2008) Molecular biology of the cell 19, 745-53. PMID: 18045989 5. Kodani, A. et al. (2009) Molecular biology of the cell 20, 1192-200. PMID: 19109421 6. Marra, P. et al. (2007) Molecular biology of the cell 18, 1595-608. PMID: 17314401
Note	For research use only.

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SDS-PAGE for Recombinant Human GM130/GOLGA2 Protein, N-His

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Datasheet

Human GM130/GOLGA2 Recombinant Protein (N-His).pdf

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Human GM130/GOLGA2 Recombinant Protein (N-His) (HC396012)

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