Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

Name: Anti-GM130/GOLGA2 Polyclonal Antibody
Brand: abinScience
SKU: HC396014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC396014

Description

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014) is a rabbit polyclonal antibody detecting GOLGA2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GM130/GOLGA2 (Ala361-Ala678).

Target

130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, Golgin subfamily A member 2, GOLGA2

Purification

Purified by antigen affinity column.

Accession

Q08379

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Golgin subfamily A member 2 (GM130/GOLGA2) is a ~113 kDa protein. Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization.

1. Puthenveedu, MA. et al. (2006) Nature cell biology 8, 238-48. PMID: 16489344

2. Wei, JH. et al. (2015) Cell 162, 287-299. PMID: 26165940

3. Rivero, S. et al. (2009) The EMBO journal 28, 1016-28. PMID: 19242490

4. Kodani, A. et al. (2008) Molecular biology of the cell 19, 745-53. PMID: 18045989

5. Kodani, A. et al. (2009) Molecular biology of the cell 20, 1192-200. PMID: 19109421

6. Marra, P. et al. (2007) Molecular biology of the cell 18, 1595-608. PMID: 17314401

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GOLGA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate

References

Datasheet

Anti-GM130/GOLGA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us