Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014)

Name: Anti-GM130/GOLGA2 Polyclonal Antibody
Brand: abinScience
SKU: HC396014
Price: 96 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC396014

Description

Anti-GM130/GOLGA2 Polyclonal Antibody (HC396014) is a rabbit polyclonal antibody detecting GOLGA2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human GM130/GOLGA2 (Ala361-Ala678).

Cible

130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, Golgin subfamily A member 2, GOLGA2

Purification

Purified by antigen affinity column.

Numéro d'accès

Q08379

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Golgin subfamily A member 2 (GM130/GOLGA2) is a ~113 kDa protein. Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization.

1. Puthenveedu, MA. et al. (2006) Nature cell biology 8, 238-48. PMID: 16489344

2. Wei, JH. et al. (2015) Cell 162, 287-299. PMID: 26165940

3. Rivero, S. et al. (2009) The EMBO journal 28, 1016-28. PMID: 19242490

4. Kodani, A. et al. (2008) Molecular biology of the cell 19, 745-53. PMID: 18045989

5. Kodani, A. et al. (2009) Molecular biology of the cell 20, 1192-200. PMID: 19109421

6. Marra, P. et al. (2007) Molecular biology of the cell 18, 1595-608. PMID: 17314401

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GOLGA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate

Reférences

Fiche technique

Anti-GM130/GOLGA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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