Anti-GRB2 Polyclonal Antibody (HX194014)

Name: Anti-GRB2 Polyclonal Antibody
Brand: abinScience
SKU: HX194014
Price: 168 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HX194014

Descripción

Anti-GRB2 Polyclonal Antibody (HX194014) is a rabbit polyclonal antibody detecting GRB2 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human GRB2 (Met1-Val217).

Objetivo

Protein Ash, GRB2, Growth factor receptor-bound protein 2, SH2/SH3 adapter GRB2, ASH, Adapter protein GRB2

Purificación

Purified by antigen affinity column.

Número de acceso

P62993

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Growth factor receptor-bound protein 2 (GRB2) is a ~25 kDa protein. Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation.

1. Li, S. et al. (2001) The EMBO journal 20, 6793-804. PMID: 11726515

2. Zhou, J. et al. (2023) Cell communication and signaling : CCS 21, 224. PMID: 37626338

3. Hou, B. et al. (2022) Cell death discovery 8, 319. PMID: 35831301

4. Montero-Vergara, J. et al. (2024) Cell death & disease 15, 14. PMID: 38182563

5. Sandouk, A. et al. (2023) Scientific reports 13, 3505. PMID: 36864087

6. Zhang, W. et al. (1998) Cell 92, 83-92. PMID: 9489702

7. Wu, L. et al. (2002) The Journal of biological chemistry 277, 40420-7. PMID: 12171928

8. Bilal, MY. et al. (2015) Frontiers in immunology 6, 141. PMID: 25870599

10. Vanshylla, K. et al. (2018) Scientific reports 8, 4244. PMID: 29523808

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-GRB2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo