ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
27.37 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Protein Ash, GRB2, Growth factor receptor-bound protein 2, SH2/SH3 adapter GRB2, ASH, Adapter protein GRB2
Background
Growth factor receptor-bound protein 2 (GRB2) is a ~25 kDa protein. Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation.
1. Li, S. et al. (2001) The EMBO journal 20, 6793-804. PMID: 11726515
2. Zhou, J. et al. (2023) Cell communication and signaling : CCS 21, 224. PMID: 37626338
3. Hou, B. et al. (2022) Cell death discovery 8, 319. PMID: 35831301
4. Montero-Vergara, J. et al. (2024) Cell death & disease 15, 14. PMID: 38182563
5. Sandouk, A. et al. (2023) Scientific reports 13, 3505. PMID: 36864087
6. Zhang, W. et al. (1998) Cell 92, 83-92. PMID: 9489702
7. Wu, L. et al. (2002) The Journal of biological chemistry 277, 40420-7. PMID: 12171928
8. Bilal, MY. et al. (2015) Frontiers in immunology 6, 141. PMID: 25870599
10. Vanshylla, K. et al. (2018) Scientific reports 8, 4244. PMID: 29523808
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human GRB2 Protein, N-His
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