Anti-GRB2 Polyclonal Antibody (HX194014)

Name: Anti-GRB2 Polyclonal Antibody
Brand: abinScience
SKU: HX194014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX194014

Description

Anti-GRB2 Polyclonal Antibody (HX194014) is a rabbit polyclonal antibody detecting GRB2 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GRB2 (Met1-Val217).

Target

Protein Ash, GRB2, Growth factor receptor-bound protein 2, SH2/SH3 adapter GRB2, ASH, Adapter protein GRB2

Purification

Purified by antigen affinity column.

Accession

P62993

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Growth factor receptor-bound protein 2 (GRB2) is a ~25 kDa protein. Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation.

1. Li, S. et al. (2001) The EMBO journal 20, 6793-804. PMID: 11726515

2. Zhou, J. et al. (2023) Cell communication and signaling : CCS 21, 224. PMID: 37626338

3. Hou, B. et al. (2022) Cell death discovery 8, 319. PMID: 35831301

4. Montero-Vergara, J. et al. (2024) Cell death & disease 15, 14. PMID: 38182563

5. Sandouk, A. et al. (2023) Scientific reports 13, 3505. PMID: 36864087

6. Zhang, W. et al. (1998) Cell 92, 83-92. PMID: 9489702

7. Wu, L. et al. (2002) The Journal of biological chemistry 277, 40420-7. PMID: 12171928

8. Bilal, MY. et al. (2015) Frontiers in immunology 6, 141. PMID: 25870599

10. Vanshylla, K. et al. (2018) Scientific reports 8, 4244. PMID: 29523808

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GRB2 polyclonal antibody at 1ug/mL on recombinant GRB2 (Catalog No. HX194012).

References

Datasheet

Anti-GRB2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-GRB2 Polyclonal Antibody (HX194014)

Overview

Images

References

Datasheet

SDS

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