Anti-GRB2 Polyclonal Antibody (HX194014)

Name: Anti-GRB2 Polyclonal Antibody
Brand: abinScience
SKU: HX194014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HX194014

Описание

Anti-GRB2 Polyclonal Antibody (HX194014) is a rabbit polyclonal antibody detecting GRB2 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human GRB2 (Met1-Val217).

Цель

Protein Ash, GRB2, Growth factor receptor-bound protein 2, SH2/SH3 adapter GRB2, ASH, Adapter protein GRB2

Очистка

Purified by antigen affinity column.

Номер доступа

P62993

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Growth factor receptor-bound protein 2 (GRB2) is a ~25 kDa protein. Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation.

1. Li, S. et al. (2001) The EMBO journal 20, 6793-804. PMID: 11726515

2. Zhou, J. et al. (2023) Cell communication and signaling : CCS 21, 224. PMID: 37626338

3. Hou, B. et al. (2022) Cell death discovery 8, 319. PMID: 35831301

4. Montero-Vergara, J. et al. (2024) Cell death & disease 15, 14. PMID: 38182563

5. Sandouk, A. et al. (2023) Scientific reports 13, 3505. PMID: 36864087

6. Zhang, W. et al. (1998) Cell 92, 83-92. PMID: 9489702

7. Wu, L. et al. (2002) The Journal of biological chemistry 277, 40420-7. PMID: 12171928

8. Bilal, MY. et al. (2015) Frontiers in immunology 6, 141. PMID: 25870599

10. Vanshylla, K. et al. (2018) Scientific reports 8, 4244. PMID: 29523808

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-GRB2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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