Anti-Human GRP78/HSPA5 Recombinant Antibody (SAA2389) (HY489013)

Name: Anti-Human GRP78/HSPA5 Recombinant Antibody (SAA2389)
Brand: abinScience
SKU: HY489013
Price: 126 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY489013

Reactividad de especies

Human

Aplicaciones

ELISA, FCM, IF, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1(L234A, L235A), kappa

ID de clon

SAA2389

Objetivo

Endoplasmic reticulum chaperone BiP, EC:3.6.4.10, 78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, Heat shock protein 70 family protein 5, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, HSPA5, GRP78

Nivel de endotoxinas

< 10 EU/mg

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P11021

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Fondo

Endoplasmic reticulum chaperone BiP (GRP78/HSPA5) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells.

1. Dana, RC. et al. (1990) Endocrinology 126, 672-4. PMID: 2294010

2. Oka, OB. et al. (2013) Molecular cell 50, 793-804. PMID: 23769672

3. Evensen, NA. et al. (2013) Journal of the National Cancer Institute 105, 1402-16. PMID: 23990668

4. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555

5. Ma, K. et al. (2002) The Journal of biological chemistry 277, 18728-35. PMID: 11907036

6. Ng, DT. et al. (1992) Molecular biology of the cell 3, 143-55. PMID: 1550958

7. Oikawa, D. et al. (2009) Experimental cell research 315, 2496-504. PMID: 19538957

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Kang, JM. et al. (2015) Cancer research 75, 3087-97. PMID: 26045166

Nota

For research use only

Isotype Control

Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)
Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-GRP78/HSPA5 Antibody (SAA2389)

Referencias

Datasheet

Anti-Human GRP78/HSPA5 Recombinant Antibody (SAA2389).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo