Anti-HSPA5 Polyclonal Antibody (HY489024)

Name: Anti-HSPA5 Polyclonal Antibody
Brand: abinScience
SKU: HY489024
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY489024

Description

Anti-HSPA5 Polyclonal Antibody (HY489024) is a rabbit polyclonal antibody detecting HSPA5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA5 (Leu351-Leu654).

Target

Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein

Purification

Purified by antigen affinity column.

Accession

P11021

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Endoplasmic reticulum chaperone BiP (HSPA5/GRP78) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells.

1. Dana, RC. et al. (1990) Endocrinology 126, 672-4. PMID: 2294010

2. Oka, OB. et al. (2013) Molecular cell 50, 793-804. PMID: 23769672

3. Evensen, NA. et al. (2013) Journal of the National Cancer Institute 105, 1402-16. PMID: 23990668

4. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555

5. Ma, K. et al. (2002) The Journal of biological chemistry 277, 18728-35. PMID: 11907036

6. Ng, DT. et al. (1992) Molecular biology of the cell 3, 143-55. PMID: 1550958

7. Oikawa, D. et al. (2009) Experimental cell research 315, 2496-504. PMID: 19538957

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Kang, JM. et al. (2015) Cancer research 75, 3087-97. PMID: 26045166

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HSPA5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: 4T1 cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Hela cell lysate
Lane 8: A549 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Mouse liver lysate
Lane11: Rat brain lysate
Lane12: Rat liver lysate