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Anti-GTPBP1 Polyclonal Antibody (HA399014)

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Aperçu
Numéro de catalogueHA399014
Description
Anti-GTPBP1 Polyclonal Antibody (HA399014) is a rabbit polyclonal antibody detecting GTPBP1 in WB, IHC, ELISA. Suitable for Human, Mouse, Rat, Bovine, and .
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsWB, IHC, ELISA
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human GTPBP1 (Thr71-Ser384).
Cible GTPBP1
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% purity as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès O00178
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsGTPBP1, GTP-binding protein 1, G-protein 1, GP-1, GP1, GTPBP1
Contexte

GTP-binding protein 1 (GTPBP1) is a ~72 kDa protein. GTPase that plays a role in the elongation phase of protein synthesis by forming ternary complexes with GTP and aminoacyl-transfer RNAs (aa-tRNAs), and delivering aa-tRNAs to the ribosomal A site in a GTP-dependent manner. Is also able to deliver deacylated tRNA to the A site. Additionally, it is involved in RNA quality control; after GTP hydrolysis, which is not immediately followed by rapid peptide bond formation, GTPBP1 likely retains aa-tRNA in the A site and promotes exosomal degradation of faulty mRNAs engaged in 80S elongation complexes. Plays a role in the regulation of circadian mRNA stability.

1. Zinoviev, A. et al. (2018) Genes & development 32, 1226-1241. PMID: 30108131
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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