Human GTPBP1 Recombinant Protein (N-His) (HA399012)

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Overview

Catalog No.	HA399012
Description	Recombinant Human GTPBP1 Protein, N-His (HA399012) expressed in E. coli, spanning Thr71-Ser384. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O00178
Protein length	Thr71-Ser384
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Predicted molecular weight	36.87 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GTPBP1, GTP-binding protein 1, G-protein 1, GP-1, GP1, GTPBP1
Background	GTP-binding protein 1 (GTPBP1) is a ~72 kDa protein. GTPase that plays a role in the elongation phase of protein synthesis by forming ternary complexes with GTP and aminoacyl-transfer RNAs (aa-tRNAs), and delivering aa-tRNAs to the ribosomal A site in a GTP-dependent manner. Is also able to deliver deacylated tRNA to the A site. Additionally, it is involved in RNA quality control; after GTP hydrolysis, which is not immediately followed by rapid peptide bond formation, GTPBP1 likely retains aa-tRNA in the A site and promotes exosomal degradation of faulty mRNAs engaged in 80S elongation complexes. Plays a role in the regulation of circadian mRNA stability. 1. Zinoviev, A. et al. (2018) Genes & development 32, 1226-1241. PMID: 30108131
Note	For research use only.

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SDS-PAGE for Recombinant Human GTPBP1 Protein, N-His

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Datasheet

Human GTPBP1 Recombinant Protein (N-His).pdf

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Human GTPBP1 Recombinant Protein (N-His) (HA399012)

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