Anti-Human Granzyme B Polyclonal Antibody (HY574014)

Name: Anti-Human Granzyme B Polyclonal Antibody
Brand: abinScience
SKU: HY574014
Price: 168 USD
Availability: InStock

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概要
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概要

カタログ番号

HY574014

説明

Anti-GZMB Polyclonal Antibody (HY574014) is a rabbit polyclonal antibody detecting GZMB in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human GZMB (Ile21-Arg246).

ターゲット

Granzyme-B, Cathepsin G-like 1, Granzyme-2, GZMB, grzmB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, CTLA1, GRB, C11, Cytotoxic T-lymphocyte proteinase 2, SECT

精製

Purified by antigen affinity column.

アクセッション番号

P10144

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis.

1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927

2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682

3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427

4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716

5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257

6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940

7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092

注意事項

For research use only.

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参考文献

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