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Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014)

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Aperçu
Numéro de catalogueVK140014
Description
Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014) is a rabbit polyclonal antibody detecting Minor capsid protein VP1. Suitable for Primate bocaparvovirus 1.
Reactivité des espècesPrimate bocaparvovirus 1 (Human bocavirus 1 type 1) (HBoV1)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant HBoV1 Minor capsid protein VP1 (Lys181-Gly290).
Cible EC:3.1.1.4, Minor capsid protein VP1, VP1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q3YPH4
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Minor capsid protein VP1 is a ~75 kDa protein. Capsid proteins self-assembles to form an icosahedral capsid with a T=1 symmetry, about 26 nm in diameter, and consisting of 60 copies of three size variants of the capsid proteins, VP1, and VP3, which differ by the presence of an N-terminal extension in the minor protein VP1 (Probable). The capsid has a channel at the 5-fold axis and there are densities extending the 5-fold axis into the interior of the capsid. The capsid encapsulates the genomic ssDNA (Probable). Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region. The additional N-terminal region of isoform Minor capsid protein VP1, called VP1u, may serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.

1. Mietzsch, M. et al. (2017) Journal of virology 91. PMID: 28331084
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody

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