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Обзор
Каталожный номерHW293014
Описание
Anti-HCFC1 Polyclonal Antibody (HW293014) is a rabbit polyclonal antibody detecting HCFC1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human HCFC1 (Asn52-His258).
Цель CFF, VCAF, HCFC1, HCF-1, HCF1, HFC1, C1 factor, HCF, VP16 accessory protein, Host cell factor 1
Очистка Purified by antigen affinity column.
Номер доступа P51610
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Host cell factor 1 (HCFC1) is a ~208 kDa protein. Transcriptional coregulator. Serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators. Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2.

1. Vinckevicius, A. et al. (2015) Molecular and cellular biology 35, 4135-46. PMID: 26416877
2. Mahajan, SS. et al. (2000) Molecular and cellular biology 20, 919-28. PMID: 10629049
3. Scarr, RB. et al. (2000) Molecular and cellular biology 20, 3568-75. PMID: 10779346
4. Khurana, B. et al. (2004) The Journal of biological chemistry 279, 33673-83. PMID: 15190068
5. Vogel, JL. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6817-22. PMID: 16624878
6. Zhou, P. et al. (2013) The Journal of biological chemistry 288, 17532-43. PMID: 23629655
7. Vogel, JL. et al. (2000) The EMBO journal 19, 683-90. PMID: 10675337
8. Piluso, D. et al. (2002) The Journal of biological chemistry 277, 46799-808. PMID: 12244100
9. Luciano, RL. et al. (2003) The Journal of biological chemistry 278, 51116-24. PMID: 14532282
10. Wysocka, J. et al. (2003) Genes & development 17, 896-911. PMID: 12670868
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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