ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
25.28 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Host cell factor 1 (HCFC1) is a ~208 kDa protein. Transcriptional coregulator. Serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators. Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2.
1. Vinckevicius, A. et al. (2015) Molecular and cellular biology 35, 4135-46. PMID: 26416877
2. Mahajan, SS. et al. (2000) Molecular and cellular biology 20, 919-28. PMID: 10629049
3. Scarr, RB. et al. (2000) Molecular and cellular biology 20, 3568-75. PMID: 10779346
4. Khurana, B. et al. (2004) The Journal of biological chemistry 279, 33673-83. PMID: 15190068
5. Vogel, JL. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6817-22. PMID: 16624878
6. Zhou, P. et al. (2013) The Journal of biological chemistry 288, 17532-43. PMID: 23629655
7. Vogel, JL. et al. (2000) The EMBO journal 19, 683-90. PMID: 10675337
8. Piluso, D. et al. (2002) The Journal of biological chemistry 277, 46799-808. PMID: 12244100
9. Luciano, RL. et al. (2003) The Journal of biological chemistry 278, 51116-24. PMID: 14532282
10. Wysocka, J. et al. (2003) Genes & development 17, 896-911. PMID: 12670868
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human HCFC1 Protein, N-His
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