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Anti-HCFC1 Polyclonal Antibody (HW293014)

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Overview
Catalog No.HW293014
Description
Anti-HCFC1 Polyclonal Antibody (HW293014) is a rabbit polyclonal antibody detecting HCFC1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HCFC1 (Asn52-His258).
Target CFF, VCAF, HCFC1, HCF-1, HCF1, HFC1, C1 factor, HCF, VP16 accessory protein, Host cell factor 1
Purification Purified by antigen affinity column.
Accession P51610
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Host cell factor 1 (HCFC1) is a ~208 kDa protein. Transcriptional coregulator. Serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators. Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2.

1. Vinckevicius, A. et al. (2015) Molecular and cellular biology 35, 4135-46. PMID: 26416877
2. Mahajan, SS. et al. (2000) Molecular and cellular biology 20, 919-28. PMID: 10629049
3. Scarr, RB. et al. (2000) Molecular and cellular biology 20, 3568-75. PMID: 10779346
4. Khurana, B. et al. (2004) The Journal of biological chemistry 279, 33673-83. PMID: 15190068
5. Vogel, JL. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6817-22. PMID: 16624878
6. Zhou, P. et al. (2013) The Journal of biological chemistry 288, 17532-43. PMID: 23629655
7. Vogel, JL. et al. (2000) The EMBO journal 19, 683-90. PMID: 10675337
8. Piluso, D. et al. (2002) The Journal of biological chemistry 277, 46799-808. PMID: 12244100
9. Luciano, RL. et al. (2003) The Journal of biological chemistry 278, 51116-24. PMID: 14532282
10. Wysocka, J. et al. (2003) Genes & development 17, 896-911. PMID: 12670868
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HCFC1 Polyclonal Antibody

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