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Anti-HMPV M2/Protein M2-2 Polyclonal Antibody (VK420014)

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Vista general
Número de catálogoVK420014
Reactividad de especiesHuman metapneumovirus (HMPV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant HMPV M2/Protein M2-2 (Thr2-Ser71).
Objetivo Protein M2-2, M2
Purificación Purified by antigen affinity column.
Número de acceso Q6WB96
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Protein M2-2 is a ~8 kDa protein. Mediates the regulatory switch from transcription to RNA replication. Acts late in infection by inhibiting viral transcription and up-regulating RNA replication. Plays a major role in antagonizing the type I IFN-mediated antiviral response. Interacts with host MAVS and prevents the interaction with its upstream partner RIGI in the signaling pathway leading to interferon production. Inhibits host RIGI signaling by preventing TRIM25-mediated RIGI ubiquitination.

1. Ren, J. et al. (2012) Journal of virology 86, 13049-61. PMID: 23015697
2. Tanaka, Y. et al. (2022) Frontiers in immunology 13, 970750. PMID: 36045682
3. Ren, J. et al. (2014) PloS one 9, e91865. PMID: 24618691
4. Kitagawa, Y. et al. (2017) Journal of virology 91. PMID: 28768858
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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