HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His) (VK420012)

Name: HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: VK420012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK420012
Description	Recombinant HMPV M2/Protein M2-2 Protein, N-GST & C-His (VK420012) expressed in E. coli, spanning Thr2-Ser71. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6WB96
Protein length	Thr2-Ser71
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human metapneumovirus (HMPV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.24 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein M2-2, M2
Background	Protein M2-2 is a ~8 kDa protein. Mediates the regulatory switch from transcription to RNA replication. Acts late in infection by inhibiting viral transcription and up-regulating RNA replication. Plays a major role in antagonizing the type I IFN-mediated antiviral response. Interacts with host MAVS and prevents the interaction with its upstream partner RIGI in the signaling pathway leading to interferon production. Inhibits host RIGI signaling by preventing TRIM25-mediated RIGI ubiquitination. 1. Ren, J. et al. (2012) Journal of virology 86, 13049-61. PMID: 23015697 2. Tanaka, Y. et al. (2022) Frontiers in immunology 13, 970750. PMID: 36045682 3. Ren, J. et al. (2014) PloS one 9, e91865. PMID: 24618691 4. Kitagawa, Y. et al. (2017) Journal of virology 91. PMID: 28768858
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant HMPV M2/Protein M2-2 Protein, N-GST & C-His

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Datasheet

HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His).pdf

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HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His) (VK420012)

Overview

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References

Datasheet

SDS

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