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Anti-HMPV M2/Protein M2-2 Polyclonal Antibody (VK420014)

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Aperçu
Numéro de catalogueVK420014
Reactivité des espècesHuman metapneumovirus (HMPV)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant HMPV M2/Protein M2-2 (Thr2-Ser71).
Cible Protein M2-2, M2
Purification Purified by antigen affinity column.
Numéro d'accès Q6WB96
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein M2-2 is a ~8 kDa protein. Mediates the regulatory switch from transcription to RNA replication. Acts late in infection by inhibiting viral transcription and up-regulating RNA replication. Plays a major role in antagonizing the type I IFN-mediated antiviral response. Interacts with host MAVS and prevents the interaction with its upstream partner RIGI in the signaling pathway leading to interferon production. Inhibits host RIGI signaling by preventing TRIM25-mediated RIGI ubiquitination.

1. Ren, J. et al. (2012) Journal of virology 86, 13049-61. PMID: 23015697
2. Tanaka, Y. et al. (2022) Frontiers in immunology 13, 970750. PMID: 36045682
3. Ren, J. et al. (2014) PloS one 9, e91865. PMID: 24618691
4. Kitagawa, Y. et al. (2017) Journal of virology 91. PMID: 28768858
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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