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Anti-HPV18 E7/Protein E7 Polyclonal Antibody
Anti-HPV18 E7/Protein E7 Polyclonal Antibody
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерVK556024
Описание
Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024) is a rabbit polyclonal antibody detecting Protein E7 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman papillomavirus 18 (HPV18)
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant HPV18 E7/Protein E7 (Met1-Gln105).
Проверенные приложения WB
Цель Protein E7, E7
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P06788
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Protein E7 is a ~11 kDa protein. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation.

1. Lau, L. et al. (2015) Science (New York, N.Y.) 350, 568-71. PMID: 26405230
Примечание For research use only
Изображения
  • Anti-HPV18 E7/Protein E7 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-E7 protein polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HPV18 E7 (Catalog No: VK556062)

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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