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Anti-HSP90B1 Polyclonal Antibody (HB250014)

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Vista general
Número de catálogoHB250014
Descripción
Anti-HSP90B1 Polyclonal Antibody (HB250014) is a rabbit polyclonal antibody detecting HSP90B1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HSP90B1 (Asp22-Ala311).
Objetivo 94 kDa glucose-regulated protein, HSP90B1, gp96 homolog, GRP94, Heat shock protein 90 kDa beta member 1, Endoplasmin, TRA1, Tumor rejection antigen 1, GRP-94
Purificación Purified by antigen affinity column.
Número de acceso P14625
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Endoplasmin (HSP90B1/GRP94) is a ~92 kDa protein. ATP-dependent chaperone involved in the processing of proteins in the endoplasmic reticulum, regulating their transport. Together with MESD, acts as a modulator of the Wnt pathway by promoting the folding of LRP6, a coreceptor of the canonical Wnt pathway. When associated with CNPY3, required for proper folding of Toll-like receptors. Promotes folding and trafficking of TLR4 to the cell surface. May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10.

1. Liu, B. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 6877-82. PMID: 23572575
2. Ma, M. et al. (2024) Science (New York, N.Y.) 386, 667-672. PMID: 39509507
3. Randow, F. et al. (2001) Nature cell biology 3, 891-6. PMID: 11584270
4. Zhang, M. et al. (2020) Cell 181, 637-652.e15. PMID: 32272059
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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