Human HSP90B1 Recombinant Protein (N-His) (HB250012)

Name: Human HSP90B1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB250012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB250012
Description	Recombinant Human HSP90B1 Protein, N-His (HB250012) expressed in E. coli, spanning Asp22-Ala311. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P14625
Protein length	Asp22-Ala311
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.91 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	94 kDa glucose-regulated protein, HSP90B1, gp96 homolog, GRP94, Heat shock protein 90 kDa beta member 1, Endoplasmin, TRA1, Tumor rejection antigen 1, GRP-94
Background	Endoplasmin (HSP90B1/GRP94) is a ~92 kDa protein. ATP-dependent chaperone involved in the processing of proteins in the endoplasmic reticulum, regulating their transport. Together with MESD, acts as a modulator of the Wnt pathway by promoting the folding of LRP6, a coreceptor of the canonical Wnt pathway. When associated with CNPY3, required for proper folding of Toll-like receptors. Promotes folding and trafficking of TLR4 to the cell surface. May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10. 1. Liu, B. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 6877-82. PMID: 23572575 2. Ma, M. et al. (2024) Science (New York, N.Y.) 386, 667-672. PMID: 39509507 3. Randow, F. et al. (2001) Nature cell biology 3, 891-6. PMID: 11584270 4. Zhang, M. et al. (2020) Cell 181, 637-652.e15. PMID: 32272059
Note	For research use only.

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SDS-PAGE for Recombinant Human HSP90B1 Protein, N-His

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Datasheet

Human HSP90B1 Recombinant Protein (N-His).pdf

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Human HSP90B1 Recombinant Protein (N-His) (HB250012)

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