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Обзор
Каталожный номерHA966014
Описание
Anti-Human ADGRG3 Polyclonal Antibody (HA966014) is a rabbit polyclonal antibody detecting ADGRG3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human ADGRG3 (Gln21-Ser272).
Цель ADGRG3, PGR26, G-protein coupled receptor 97, Adhesion G protein-coupled receptor G3, GPR97, G-protein coupled receptor PGR26
Очистка Purified by antigen affinity column.
Номер доступа Q86Y34
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Adhesion G protein-coupled receptor G3 (ADGRG3) is a ~60 kDa protein. Adhesion G-protein coupled receptor (aGPCR) for glucocorticoid hormones such as cortisol, cortisone and 11-deoxycortisol. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. ADGRG3/GPR97 is coupled to G(o)/GNAO1 G proteins and mediates signaling by inhibiting adenylate cyclase activity. May also signal through G-alpha(q)-proteins; additional evidence are however required to confirm this result in vivo. Plays a role in the regulation of various processes including B-cell development, inflammation or innate immunity.

1. Ping, YQ. et al. (2021) Nature 589, 620-626. PMID: 33408414
2. Gupte, J. et al. (2012) FEBS letters 586, 1214-9. PMID: 22575658
3. Hsiao, CC. et al. (2018) Frontiers in immunology 9, 2830. PMID: 30559745
4. Chu, TY. et al. (2022) Nature communications 13, 6385. PMID: 36302784
5. Valtcheva, N. et al. (2013) The Journal of biological chemistry 288, 35736-48. PMID: 24178298
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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