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Anti-Human ADGRG3 Polyclonal Antibody (HA966024)

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Aperçu
Numéro de catalogueHA966024
Description
Anti-Human ADGRG3 Polyclonal Antibody (HA966024) is a rabbit polyclonal antibody detecting ADGRG3. Suitable for Human.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ADGRG3 (2*(Gly396-Thr434)).
Cible ADGRG3, ADGRG3 C-terminal fragment, ADGRG3 N-terminal fragment, Adhesion G protein-coupled receptor G3, Adhesion G-protein coupled receptor G3, C-terminal fragment, Adhesion G-protein coupled receptor G3, N-terminal fragment, G-protein coupled receptor 97, GPR97, PGR26
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q86Y34
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Adhesion G protein-coupled receptor G3 (ADGRG3) is a ~60 kDa protein. Adhesion G-protein coupled receptor (aGPCR) for glucocorticoid hormones such as cortisol, cortisone and 11-deoxycortisol. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. ADGRG3/GPR97 is coupled to G(o)/GNAO1 G proteins and mediates signaling by inhibiting adenylate cyclase activity. May also signal through G-alpha(q)-proteins; additional evidence are however required to confirm this result in vivo. Plays a role in the regulation of various processes including B-cell development, inflammation or innate immunity.

1. Ping, YQ. et al. (2021) Nature 589, 620-626. PMID: 33408414
2. Gupte, J. et al. (2012) FEBS letters 586, 1214-9. PMID: 22575658
3. Hsiao, CC. et al. (2018) Frontiers in immunology 9, 2830. PMID: 30559745
4. Chu, TY. et al. (2022) Nature communications 13, 6385. PMID: 36302784
5. Valtcheva, N. et al. (2013) The Journal of biological chemistry 288, 35736-48. PMID: 24178298
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ADGRG3 Polyclonal Antibody

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