Anti-Human ANKZF1 Recombinant Antibody (SAA2125) (HA094013)

Name: Anti-Human ANKZF1 Recombinant Antibody (SAA2125)
Brand: abinScience
SKU: HA094013
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HA094013

Reactividad de especies

Human

Aplicaciones

ELISA, IF, IP

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a

ID de clon

SAA2125

Objetivo

ANKZF1, ZNF744, Ankyrin repeat and zinc finger domain containing 1, Ankyrin repeat and zinc finger domain-containing protein 1, Zinc finger protein 744

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q9H8Y5

RRID

Anti-Human ANKZF1 Antibody (SAA2125) (abinScience Cat# HA094013, RRID:AB_3729477)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

tRNA endonuclease ANKZF1 is a ~80 kDa protein. Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation. The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts. ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs. ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1. Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress.

1. Verma, R. et al. (2018) Nature 557, 446-451. PMID: 29632312

2. Kuroha, K. et al. (2018) Molecular cell 72, 286-302.e8. PMID: 30244831

3. Yip, MCJ. et al. (2019) Nature structural & molecular biology 26, 343-349. PMID: 31011209

4. Yip, MCJ. et al. (2020) Cell reports 30, 2106-2114.e5. PMID: 32075755

5. van Haaften-Visser, DY. et al. (2017) The Journal of biological chemistry 292, 7904-7920. PMID: 28302725

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human ANKZF1 Antibody (SAA2125)

Referencias

Datasheet

Anti-Human ANKZF1 Recombinant Antibody (SAA2125).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo