Anti-Human ANKZF1 Recombinant Antibody (SAA2126) (HA094023)

Name: Anti-Human ANKZF1 Recombinant Antibody (SAA2126)
Brand: abinScience
SKU: HA094023
Price: 191 USD
Availability: InStock

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100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA094023

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a

Clone ID

SAA2126

Target

ANKZF1, ZNF744, Ankyrin repeat and zinc finger domain containing 1, Ankyrin repeat and zinc finger domain-containing protein 1, Zinc finger protein 744

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9H8Y5

RRID

Anti-Human ANKZF1 Antibody (SAA2126) (abinScience Cat# HA094023, RRID:AB_3729478)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

tRNA endonuclease ANKZF1 is a ~80 kDa protein. Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation. The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts. ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs. ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1. Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress.

1. Verma, R. et al. (2018) Nature 557, 446-451. PMID: 29632312

2. Kuroha, K. et al. (2018) Molecular cell 72, 286-302.e8. PMID: 30244831

3. Yip, MCJ. et al. (2019) Nature structural & molecular biology 26, 343-349. PMID: 31011209

4. Yip, MCJ. et al. (2020) Cell reports 30, 2106-2114.e5. PMID: 32075755

5. van Haaften-Visser, DY. et al. (2017) The Journal of biological chemistry 292, 7904-7920. PMID: 28302725

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human ANKZF1 Antibody (SAA2126)

References

Datasheet

Anti-Human ANKZF1 Recombinant Antibody (SAA2126).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ANKZF1 Recombinant Antibody (SAA2126) (HA094023)

Overview

Images

References

Datasheet

SDS

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