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| カタログ番号 | HA094013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | ELISA, IF, IP | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a | ||||
| クローンID | SAA2125 | ||||
| ターゲット | ANKZF1, ZNF744, Ankyrin repeat and zinc finger domain containing 1, Ankyrin repeat and zinc finger domain-containing protein 1, Zinc finger protein 744 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | Q9H8Y5 | ||||
| RRID | Anti-Human ANKZF1 Antibody (SAA2125) (abinScience Cat# HA094013, RRID:AB_3729477) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | tRNA endonuclease ANKZF1 is a ~80 kDa protein. Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation. The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts. ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs. ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1. Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress. 1. Verma, R. et al. (2018) Nature 557, 446-451. PMID: 29632312 2. Kuroha, K. et al. (2018) Molecular cell 72, 286-302.e8. PMID: 30244831 3. Yip, MCJ. et al. (2019) Nature structural & molecular biology 26, 343-349. PMID: 31011209 4. Yip, MCJ. et al. (2020) Cell reports 30, 2106-2114.e5. PMID: 32075755 5. van Haaften-Visser, DY. et al. (2017) The Journal of biological chemistry 292, 7904-7920. PMID: 28302725 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Human ANKZF1 Antibody (SAA2125)
+86-027-65523339
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