Anti-Human ANKZF1 Recombinant Antibody (SAA2125) (HA094013)

Name: Anti-Human ANKZF1 Recombinant Antibody (SAA2125)
Brand: abinScience
SKU: HA094013
Price: 191 USD
Availability: InStock

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Спецификация:

50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HA094013

Реактивность видов

Human

Применения

ELISA, IF, IP

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a

ID клона

SAA2125

Цель

ANKZF1, ZNF744, Ankyrin repeat and zinc finger domain containing 1, Ankyrin repeat and zinc finger domain-containing protein 1, Zinc finger protein 744

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

Q9H8Y5

RRID

Anti-Human ANKZF1 Antibody (SAA2125) (abinScience Cat# HA094013, RRID:AB_3729477)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

tRNA endonuclease ANKZF1 is a ~80 kDa protein. Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation. The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts. ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs. ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1. Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress.

1. Verma, R. et al. (2018) Nature 557, 446-451. PMID: 29632312

2. Kuroha, K. et al. (2018) Molecular cell 72, 286-302.e8. PMID: 30244831

3. Yip, MCJ. et al. (2019) Nature structural & molecular biology 26, 343-349. PMID: 31011209

4. Yip, MCJ. et al. (2020) Cell reports 30, 2106-2114.e5. PMID: 32075755

5. van Haaften-Visser, DY. et al. (2017) The Journal of biological chemistry 292, 7904-7920. PMID: 28302725

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Human ANKZF1 Antibody (SAA2125)

Ссылки

Даташит

Anti-Human ANKZF1 Recombinant Antibody (SAA2125).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа