

| Каталожный номер | HP641014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Реактивность видов | Human, Mouse | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant Human ANO1 (Met117-Gly325). | ||||||||
| Цель | Anoctamin-1, Tumor-amplified and overexpressed sequence 2, Transmembrane protein 16A, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, TMEM16A, ANO1, TAOS2, ORAOV2, DOG1 | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | Q5XXA6 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Фон | Anoctamin-1 (ANO1/TMEM16A) is a ~114 kDa protein. Calcium-activated chloride channel (CaCC). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression. 1. Schreiber, R. et al. (2010) The Journal of biological chemistry 285, 7838-45. PMID: 20056604 2. Ousingsawat, J. et al. (2011) Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 28, 715-24. PMID: 22178883 3. Tian, Y. et al. (2012) Journal of cell science 125, 4991-8. PMID: 22946059 4. Park, JH. et al. (2021) Journal of medical genetics 58, 247-253. PMID: 32487539 5. Benedetto, R. et al. (2017) Scientific reports 7, 12397. PMID: 28963502 6. Cabrita, I. et al. (2021) American journal of respiratory cell and molecular biology 64, 50-58. PMID: 33026825 7. Simões, FB. et al. (2019) Life science alliance 2. PMID: 31732694 | ||||||||
| Примечание | For research use only. |

Western blot analysis was performed using anti-ANO1 polyclonal antibody at 1µg/ml on various samples.
Lane 1: 293T cell lysate




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