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Каталожный номерHP865014
Описание
Anti-Human ARHGEF1 Polyclonal Antibody (HP865014) is a rabbit polyclonal antibody detecting ARHGEF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human ARHGEF1 (Ser39-Gly230).
Цель p115-RhoGEF, p115RhoGEF, 115 kDa guanine nucleotide exchange factor, Sub1.5, Rho guanine nucleotide exchange factor 1, ARHGEF1
Очистка Purified by antigen affinity column.
Номер доступа Q92888
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Rho guanine nucleotide exchange factor 1 (ARHGEF1) is a ~102 kDa protein. Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.

1. Kozasa, T. et al. (1998) Science (New York, N.Y.) 280, 2109-11. PMID: 9641915
2. Hart, MJ. et al. (1998) Science (New York, N.Y.) 280, 2112-4. PMID: 9641916
3. Bouafia, A. et al. (2019) The Journal of clinical investigation 129, 1047-1060. PMID: 30521495
4. Hart, MJ. et al. (1996) The Journal of biological chemistry 271, 25452-8. PMID: 8810315
5. Guilluy, C. et al. (2010) Nature medicine 16, 183-90. PMID: 20098430
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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