Human ARHGEF1 Recombinant Protein (N-His) (HP865012)

Name: Human ARHGEF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP865012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP865012
Description	Recombinant Human ARHGEF1 Protein, N-His (HP865012) expressed in E. coli, spanning Ser39-Gly230. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q92888
Protein length	Ser39-Gly230
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.35 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	p115-RhoGEF, p115RhoGEF, 115 kDa guanine nucleotide exchange factor, Sub1.5, Rho guanine nucleotide exchange factor 1, ARHGEF1
Background	Rho guanine nucleotide exchange factor 1 (ARHGEF1) is a ~102 kDa protein. Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. 1. Kozasa, T. et al. (1998) Science (New York, N.Y.) 280, 2109-11. PMID: 9641915 2. Hart, MJ. et al. (1998) Science (New York, N.Y.) 280, 2112-4. PMID: 9641916 3. Bouafia, A. et al. (2019) The Journal of clinical investigation 129, 1047-1060. PMID: 30521495 4. Hart, MJ. et al. (1996) The Journal of biological chemistry 271, 25452-8. PMID: 8810315 5. Guilluy, C. et al. (2010) Nature medicine 16, 183-90. PMID: 20098430
Note	For research use only.

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SDS-PAGE for Recombinant Human ARHGEF1 Protein, N-His

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Datasheet

Human ARHGEF1 Recombinant Protein (N-His).pdf

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Human ARHGEF1 Recombinant Protein (N-His) (HP865012)

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