Anti-Human ARHGEF1 Polyclonal Antibody (HP865014)

Name: Anti-Human ARHGEF1 Polyclonal Antibody
Brand: abinScience
SKU: HP865014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP865014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARHGEF1 (Ser39-Gly230).

Target

p115-RhoGEF, p115RhoGEF, 115 kDa guanine nucleotide exchange factor, Sub1.5, Rho guanine nucleotide exchange factor 1, ARHGEF1

Purification

Purified by antigen affinity column.

Accession

Q92888

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Rho guanine nucleotide exchange factor 1 (ARHGEF1) is a ~102 kDa protein. Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.

1. Kozasa, T. et al. (1998) Science (New York, N.Y.) 280, 2109-11. PMID: 9641915

2. Hart, MJ. et al. (1998) Science (New York, N.Y.) 280, 2112-4. PMID: 9641916

3. Bouafia, A. et al. (2019) The Journal of clinical investigation 129, 1047-1060. PMID: 30521495

4. Hart, MJ. et al. (1996) The Journal of biological chemistry 271, 25452-8. PMID: 8810315

5. Guilluy, C. et al. (2010) Nature medicine 16, 183-90. PMID: 20098430

Note

For research use only.

Images

References

Datasheet

Anti-Human ARHGEF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ARHGEF1 Polyclonal Antibody (HP865014)

Overview

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References

Datasheet

SDS

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