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Anti-Human ASL Polyclonal Antibody (HY187014)

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Vista general
Número de catálogoHY187014
Descripción
Anti-Human ASL Polyclonal Antibody (HY187014) is a rabbit polyclonal antibody detecting ASL in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ASL (Met1-Ala464).
Objetivo ASAL, Arginosuccinase, Argininosuccinate lyase, ASL
Purificación Purified by antigen affinity column.
Número de acceso P04424
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Argininosuccinate lyase (ASL) is a ~51 kDa protein. Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway.

1. Sampaleanu, LM. et al. (2001) Biochemistry 40, 15570-80. PMID: 11747432
2. Yu, B. et al. (2001) Biochemistry 40, 15581-90. PMID: 11747433
3. Erez, A. et al. (2011) Nature medicine 17, 1619-26. PMID: 22081021
4. Walker, DC. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 9625-9. PMID: 2263616
5. Walker, DC. et al. (1997) The Journal of biological chemistry 272, 6777-83. PMID: 9045711
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ASL Polyclonal Antibody

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