Human ASL Recombinant Protein (N-His) (HY187012)

Name: Human ASL Recombinant Protein (N-His)
Brand: abinScience
SKU: HY187012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY187012
Description	Recombinant Human ASL Protein, N-His (HY187012) expressed in E. coli, spanning Met1-Ala464. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P04424
Protein length	Met1-Ala464
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	53.82 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ASAL, Arginosuccinase, Argininosuccinate lyase, ASL
Background	Argininosuccinate lyase (ASL) is a ~51 kDa protein. Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway. 1. Sampaleanu, LM. et al. (2001) Biochemistry 40, 15570-80. PMID: 11747432 2. Yu, B. et al. (2001) Biochemistry 40, 15581-90. PMID: 11747433 3. Erez, A. et al. (2011) Nature medicine 17, 1619-26. PMID: 22081021 4. Walker, DC. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 9625-9. PMID: 2263616 5. Walker, DC. et al. (1997) The Journal of biological chemistry 272, 6777-83. PMID: 9045711
Note	For research use only.

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SDS-PAGE for Recombinant Human ASL Protein, N-His

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Datasheet

Human ASL Recombinant Protein (N-His).pdf

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Human ASL Recombinant Protein (N-His) (HY187012)

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