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|---|---|---|---|---|---|---|---|
| Reactividad de especies | Human | ||||||
| Aplicaciones | ELISA, FCM, WB | ||||||
| Especie huésped | Mouse | ||||||
| Clonalidad | Monoclonal | ||||||
| Isotipo | IgG2b-kappa | ||||||
| ID de clon | BBM.1 | ||||||
| Objetivo | Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P | ||||||
| Nivel de endotoxinas | < 10 EU/mg | ||||||
| Pureza | >95% as determined by SDS-PAGE. | ||||||
| Purificación | Protein A/G purified from cell culture supernatant. | ||||||
| Número de acceso | P61769 | ||||||
| Forma | Liquid | ||||||
| Buffer de almacenamiento | 0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
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| Información de uso del producto |
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| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Nota | For research use only. | ||||||
| Isotype Control | Mouse IgG2b, kappa Recombinant Isotype Control Antibody (MPC-11) (MF974207) |

SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).

Detects B2M/Beta-2-microglobulin in indirect ELISAs.

Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).

Flow-cytometry using APC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD1a & B2M (Catalog No: HY043011)
Lane 2: negative control




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