Anti-B2M Polyclonal Antibody (HX250014)

Name: Anti-B2M Polyclonal Antibody
Brand: abinScience
SKU: HX250014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX250014

Description

Anti-B2M Polyclonal Antibody (HX250014) is a rabbit polyclonal antibody detecting B2M in ELISA, IHC, WB. Suitable for Human, Alouatta seniculus, Aotus azarae, Aotus lemurinus, and Aotus nancymaae.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human B2M (Gln22-Met119).

Target

Beta-2-microglobulin, B2M

Purification

Purified by antigen affinity column.

Accession

P61769

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Beta-2-microglobulin (B2M/β2-MG) is a ~13 kDa protein. Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation.

1. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-B2M polyclonal antibody at 1ug/mL on various samples.
Lane 1: U937 cell lysate
Lane 2: Raji cell lysate