Anti-Human beta 2-Microglobulin Recombinant Antibody (BBM.1) (HX250013)

Name: Anti-Human beta 2-Microglobulin Recombinant Antibody (BBM.1)
Brand: abinScience
SKU: HX250013
Price: 110 USD
Availability: InStock

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1mg

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Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HX250013

Reactivité des espèces

Human

Applications

ELISA, FCM, WB

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2b-kappa

ID de clone

BBM.1

Cible

Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P61769

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Note

For research use only.

Isotype Control

Mouse IgG2b, kappa Recombinant Isotype Control Antibody (MPC-11) (MF974207)

Images

SEC-HPLC

SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).
Bioactivity

Detects B2M/Beta-2-microglobulin in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).
Flow CytoMetry

Flow-cytometry using APC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD1a & B2M (Catalog No: HY043011)
Lane 2: negative control