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Anti-Human BAG3 Recombinant Antibody (SAA0529), FITC (HF640117)

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概要
カタログ番号HF640117
種反応性Human
アプリケーションELISA, FCM
宿主種Human
クローン性Monoclonal
アイソタイプIgG2a, kappa
クローンIDSAA0529
抱合FITC
ターゲット Docking protein CAIR-1, BAG3, Bcl-2-associated athanogene 3, BAG family molecular chaperone regulator 3, Bcl-2-binding protein Bis, BAG-3, BIS
エンドトキシンレベル Please contact with the lab for this information.
アクセッション番号 O95817
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

BAG family molecular chaperone regulator 3 (BAG3) is a ~61 kDa protein. Co-chaperone and adapter protein that connects different classes of molecular chaperones including heat shock proteins 70 (HSP70s), e.g. HSPA1A/HSP70 or HSPA8/HSC70, and small heat shock proteins (sHSPs), e.g. HSPB8. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from HSP70s, thereby triggering client protein release. Nucleotide release is mediated via BAG3 binding to the nucleotide-binding domain (NBD) of HSP70s, whereas client release is mediated via binding to the substrate-binding domain (SBD).

1. Rauch, JN. et al. (2017) Journal of molecular biology 429, 128-141. PMID: 27884606
2. Meister-Broekema, M. et al. (2018) Nature communications 9, 5342. PMID: 30559338
3. Rauch, JN. et al. (2016) The Journal of biological chemistry 291, 19848-57. PMID: 27474739
4. Takayama, S. et al. (1999) The Journal of biological chemistry 274, 781-6. PMID: 9873016
5. Lee, JH. et al. (1999) Oncogene 18, 6183-90. PMID: 10597216
6. Jin, YH. et al. (2015) Biochemical and biophysical research communications 464, 561-7. PMID: 26159920
注意事項 For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
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