Anti-Human BAX Recombinant Antibody (3C10) (HC491043)

Name: Anti-Human BAX Recombinant Antibody (3C10)
Brand: abinScience
SKU: HC491043
Price: 191 USD
Availability: InStock

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Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC491043

Reactivité des espèces

Human

Applications

ELISA, WB

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

3C10

Cible

BAX, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q07812

RRID

Anti-Human BAX Antibody (3C10) (abinScience Cat# HC491043, RRID:AB_3729489)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Apoptosis regulator BAX is a ~21 kDa protein. Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

1. Schmitt, E. et al. (2000) Biochemical and biophysical research communications 270, 868-79. PMID: 10772918

2. Tan, KO. et al. (2001) The Journal of biological chemistry 276, 2802-7. PMID: 11060313

3. Zhang, H. et al. (2005) Nature cell biology 7, 909-15. PMID: 16113678

4. Tan, KO. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 14623-8. PMID: 16199525

5. Gavathiotis, E. et al. (2008) Nature 455, 1076-81. PMID: 18948948

6. Czabotar, PE. et al. (2011) The Journal of biological chemistry 286, 7123-31. PMID: 21199865

7. Edlich, F. et al. (2011) Cell 145, 104-16. PMID: 21458670

8. Wei, B. et al. (2015) Journal of virology 89, 3804-18. PMID: 25609812

9. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894

10. Oltvai, ZN. et al. (1993) Cell 74, 609-19. PMID: 8358790

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human BAX Antibody (3C10)

Reférences

Fiche technique

Anti-Human BAX Recombinant Antibody (3C10).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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