Anti-Human BAX Recombinant Nanobody (SAA2133) (HC491053)

Name: Anti-Human BAX Recombinant Nanobody (SAA2133)
Brand: abinScience
SKU: HC491053
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC491053

Description

Anti-Human BAX Nanobody (SAA2133) [SAA2133] (HC491053) is a alpaca monoclonal antibody detecting BAX in ELISA, SPR. Suitable for Human.

Species reactivity

Human

Applications

ELISA, SPR

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA2133

Clonality

Monoclonal

Target

BAX, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

Q07812

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apoptosis regulator BAX is a ~21 kDa protein. Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

1. Schmitt, E. et al. (2000) Biochemical and biophysical research communications 270, 868-79. PMID: 10772918

2. Tan, KO. et al. (2001) The Journal of biological chemistry 276, 2802-7. PMID: 11060313

3. Zhang, H. et al. (2005) Nature cell biology 7, 909-15. PMID: 16113678

4. Tan, KO. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 14623-8. PMID: 16199525

5. Gavathiotis, E. et al. (2008) Nature 455, 1076-81. PMID: 18948948

6. Czabotar, PE. et al. (2011) The Journal of biological chemistry 286, 7123-31. PMID: 21199865

7. Edlich, F. et al. (2011) Cell 145, 104-16. PMID: 21458670

8. Wei, B. et al. (2015) Journal of virology 89, 3804-18. PMID: 25609812

9. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894

10. Oltvai, ZN. et al. (1993) Cell 74, 609-19. PMID: 8358790

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human BAX Nanobody (SAA2133).

References

Datasheet

Anti-Human BAX Recombinant Nanobody (SAA2133).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human BAX Recombinant Nanobody (SAA2133) (HC491053)

Overview

Images

References

Datasheet

SDS

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