Anti-Human BRD2 Polyclonal Antibody (HB192014)

Name: Anti-Human BRD2 Polyclonal Antibody
Brand: abinScience
SKU: HB192014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB192014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human BRD2 (Arg74-Gln182).

Objetivo

Really interesting new gene 3 protein, KIAA9001, O27.1.1, BRD2, RING3, Bromodomain-containing protein 2

Purificación

Purified by antigen affinity column.

Número de acceso

P25440

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Bromodomain-containing protein 2 (BRD2) is a ~88 kDa protein. Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation. Also recognizes and binds acetylated non-histone proteins, such as STAT3. Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin.

1. Nakamura, Y. et al. (2007) The Journal of biological chemistry 282, 4193-201. PMID: 17148447

2. Huang, H. et al. (2007) BMC structural biology 7, 57. PMID: 17848202

3. LeRoy, G. et al. (2008) Molecular cell 30, 51-60. PMID: 18406326

4. Umehara, T. et al. (2010) The Journal of biological chemistry 285, 7610-8. PMID: 20048151

5. Umehara, T. et al. (2010) FEBS letters 584, 3901-8. PMID: 20709061

6. Filippakopoulos, P. et al. (2010) Nature 468, 1067-73. PMID: 20871596

7. Cheung, KL. et al. (2017) Molecular cell 65, 1068-1080.e5. PMID: 28262505

8. Xie, L. et al. (2022) Nature genetics 54, 481-491. PMID: 35410381

Nota

For research use only.

Imágenes

Western blot

Lane 1: C2C12 cell lysate

Referencias

Datasheet

Anti-Human BRD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo